The reign of “Barbie” is over. As far as the box office is concerned, at least.

Despite being released on July 21, the Greta Gerwig-directed film failed to top the weekend box office for the first time since its release. According to studio estimates, “Blue Beetle” took its place and grossed an estimated $25.4 million, while “Barbie” came in second with $21.5 million.

“Oppenheimer,” which was also released the same weekend as “Barbie,” fell to third and earned $10.6 million, bringing its total US take to $285 million.

“Barbie” has grossed $567 million at the US box office so far. Several records are broken by the film as it ends on top.

Warner Bros.’ “The Dark Knight” became its highest-grossing domestic release last week, passing it by; it also is on track to surpass Universal Pictures’ “The Super Mario Bros. A Movie” as the year’s biggest domestic release. In the US, that movie has made $574.2 million since its release on April 5.

Hollywood Productions. “Barbie” also crossed the $1.2 billion mark globally last week, after hitting the $1 billion mark less than three weeks earlier — a feat that only 50 films have accomplished, adjusted for inflation, according to Paul Dergarabedian, Comscore senior media analyst. Gerwig now holds the record for being the highest-grossing woman director of all time.